I have MS-DOS Boot disk, and I have no problems

I have 624KB of Base memory with that
one of the "magic" instructions in config.sys:
devicehigh=a:\dostools\emm386.exe RAM D=48
What you have to do is create a boot disk in control panel-> system.
then you change your autoexec.bat e config.sys, I give my examples, not equal to yours for sure but its similar:
Autoexec.bat:
@echo off
SET DOS_SBE=A220 I5 D1 H5 P330 T6
SET PATH=C:\WINDOWS;C:\WINDOWS\COMMAND;a:\DOSTOOLS\SBDOS;a:\DOSTOOLS
SET BLASTER=A220 I5 D1 H5 P330 T6
SET CTSYN=c:\windows
lh a:\DOSTOOLS\SBDOS\SBEINIT.COM > c:\sblive.txt
rem lh a:\dostools\MSCDEX.EXE /D:warlock /V /M:1
lh a:\mouse\ctm-pt.exe > c:\mouse.txt
rem keyb po,,C:\WINDOWS\COMMAND\keyboard.sys
lh keyb po
Config.sys:
files=60
DOS=HIGH,UMB
Device=a:\dostools\Himem.Sys
devicehigh=a:\dostools\emm386.exe RAM D=48
rem devicehigh=a:\dostools\ibmidecd.sys /D:warlock
rem device=C:\WINDOWS\COMMAND\display.sys con=(ega,,1)
rem Country=351,850,C:\WINDOWS\COMMAND\country.sys
But one thing that help its to have mouse and CDrom drivers that dont spend much memory, I use ctm for mouse driver and it spends only 3 KB!!
Running:
Creative SB16 Emulation Driver, Version 4.05
Copyright(c) 1996-2000, Creative Technology Ltd., All Rights Reserved.
Creative Audio @ Port a000, IRQ 10
Initialization Complete.
CuteMouse v1.9 [FreeDOS]
Instalado em porta PS/2
MEM:
Nome Total / Convencional / Superior Memory
-------- ---------------- ---------------- ----------------
SYSTEM 21,072 (21K) / 10,896 (11K) / 10,176 (10K)
HIMEM 1,168 (1K) / 1,168 (1K) / 0 (0K)
EMM386 4,320 (4K) / 4,320 (4K) / 0 (0K)
COMMAND 7,168 (7K) / 0 (0K) / 7,168 (7K)
SBEINIT 4,736 (5K) / 0 (0K) / 4,736 (5K)
CTM-PT 3,328 (3K) / 0 (0K) / 3,328 (3K)
KEYB 6,944 (7K) / 0 (0K) / 6,944 (7K)
Free 678,640 (663K) 638,736 (624K) 39,904 (39K)
MEM Resume
Tipo de memória Total Used Free
---------------- ----------- ----------- -----------
Convencional 655,360 16,624 638,736
Superior 72,256 32,352 39,904
---------------- ----------- ----------- -----------
Total de memória 536,395,776 4,028,688 532,367,088
Tamanho do maior programa executável 638,720 (624K)
Maior bloco de memória superior livre 39,552 (39K)