**
Potential Pitfalls
I did a post discussing the map design problems with
understanding that tiles are single pixels even in game. One fundamental problem is that in .bmp the pixels are a square grid, while the Civ map uses a diamond shaped grid. Here's a post ,with discussion following on in further posts, illustrating the
problems with converting a square, one pixel over another, image into the isometric tile-shaped maps of Civ. To get a decently shaped map the image had to be stretched taller, empty ocean colored bands added to make it square, then the empty ocean had to be cropped off, either in Map Tweaker or the editor, to make a usable map.
Grid Overlay Method
This
Map Making thread has a good discussion of how to do a decent freehand map & works very well if the map is already a digital image, as you have. Be sure to read all two pages because several variations are discussed and the best ones (IMHO) come last. The biggest advantage is that you can do all kinds of shapes & sizes of maps. & The method doesn't depend on
your level of artistic skill - just starting with a decent map & patient application of effort.