According to the college-level textbook Biology by Neil Campbell and Jane Reece, Biosphere 2 suffered from CO2 levels that "fluctuated wildly" and that most of the vertebrate species and all of the pollinating insects died. Insect pests, like cockroaches, boomed. In practice, ants, a companion to one of the tree species (Cecropia) in the Rain Forest, had been introduced. By 1993 the tramp ant species Paratrechina longicornis, local to the area had been unintentionally sealed in and had come to dominate.[7] Galagos reproduced in Biosphere 2, but a number of pollinating insects were lost to ant predation and several bird species were lost.
During the first mission, the oxygen inside the facility, which began at 20.9%, fell at a steady pace and after 16 months was down to 14.5%. This is equivalent to the oxygen availability at an elevation of 4,080 meters (13,400 ft).[14] Since some biospherians were starting to have symptoms like sleep apnea and fatigue, Walford and the medical team decided to boost oxygen with injections in January and August 1993.
Daily fluctuation of carbon dioxide dynamics was typically 600 ppm because of the strong drawdown during sunlight hours by plant photosynthesis, followed by a similar rise during the nighttime when system respiration dominated. As expected, there was also a strong seasonal signature to CO2 levels, with wintertime levels as high as 4000-4500 and summertime levels near 1000 ppm. The crew worked to manage the CO2 by occasionally turning on a CO2 scrubber, activating and de-activating the desert and savannah through control of irrigation water, cutting and storing biomass to sequester carbon, and utilizing all potential planting areas with fast-growing species to increase system photosynthesis. [15]
Many suspected the drop in oxygen was due to microbes in the soil. The soils were selected to have enough carbon to provide for the plants of the ecosystems to grow from infancy to maturity, a plant mass increase of perhaps 20 tons.[16] The release rate of that soil carbon as carbon dioxide by respiration of soil microbes was an unknown that the Biosphere 2 experiment was designed to reveal.
The respiration rate was faster than the photosynthesis (possibly in part due to relatively low light penetration through the glazed structure) resulting in a slow decrease of oxygen. A mystery accompanied the oxygen decline: the corresponding increase in carbon dioxide did not appear. This concealed the underlying process until an investigation by Jeff Severinghaus and Wallace Broecker of Columbia University’s Lamont Doherty Earth Observatory using isotopic analysis showed that carbon dioxide was reacting with exposed concrete inside Biosphere 2 to form calcium carbonate, thereby sequestering the carbon dioxide and, as part of it, the oxygen that had disappeared. [17]
The discovery of the small difference between rate of respiration and rate of photosynthesis depended on the extremely low leak rate of Biosphere 2. It was shown by Dempster that had Biosphere 2 leaked as much as other closed ecological test chambers, the wash-out effect of outside air mixing in would have concealed the entire imbalance.