Quick! Hit ctrl(or command)+v, number 2

Plasmids are circular DNA fragments within bacteria that replicate autonomously from the organism. As such, plasmids can be physically modified in order to put foreign strands of DNA in them, thus changing the code and subsequent phenotypal result of the plasmid. In order to insert the wanted gene into the plasmid, the plasmid must first be cut by a specific restriction enzyme that will only cut the plasmid in one location to prevent it from destroying itself. This restriction enzyme will form a specific pattern of sticky ends on the cut plasmid. It is important to note that every restriction enzyme will code to a different sticky end and that each set of sticky ends are complementary to only each other. After that, the restriction enzyme will cut the DNA with the same sticky end pattern on both sides of the wanted gene, and insert the gene into the plasmid, sticking the ends together using the enzyme ligase, thus once more making the plasmid circular. A simple, modular method for determining which bacterial cells have been successfully transformed is to, regarding the type of gene used, specifically apply certain substances that with the influence of the gene will be modified and constructed into another substance, or to inflict a certain condition or status that can be detected by and reacted to by a specific gene and its derivatives. To do this, however, it is necessary to know exactly what the original gene will code for. After applying the certain status or substances, it is then simple to test which cells produce product or react, and which do not.
 
English Research Paper:
Library Systems and Resource Searches

Objectives Four-Nine

Distributed: Wednesday, February 8, 2012 Due: Thursday, February 16, 2012

 The Research Paper requires the student to demonstrate both familiarity with the library systems and the ability to use each system to search for resource materials.

 List your “Interest Area”:




 CONDUCT the following resource searches for your “Interest Area” (IA).

 RECORD the findings below.

 PRINT a copy of each search result and PRINT your name – period numeral in the upper right-hand corner of each page.

Objective Four: Proquest Research Library (Periodicals) helps researchers understand the crucial issues in business, politics, government, education, and popular culture:

Works Cited #1:


Works Cited #2:


Objective Five: Issues: Understanding Controversy and Society provides in-depth and carefully selected primary and secondary sources articles on today's most important issues:

Works Cited #1:


Works Cited #2:


Objective Six: Opposing Viewpoints in Context covers social issues and contains a broad variety of data for conducting research:

Works Cited #1:


Works Cited #2


English Research Paper: Library Systems and Resource Searches Objectives Four – Nine 2

Objective Seven: Points of View examines controversial issues in national and international news:

Works Cited #1:


Works Cited #2:


Objective Eight: SIRS Issues Researcher presents the historical origins, related issues, global perspectives, and the essential questions surrounding controversial topics:

Works Cited #1:


Works Cited #2:


Objective Nine: Congressional Digest Debates provides an impartial source for information on controversial topics – with no agenda beyond the promotion of independent thought and a well-informed electorate (Required for 9H Only):

Works Cited #1:


Works Cited #2:


The student must review this information with his/her parent/guardian and both must sign below to signify that this review has taken place.


___________________________________ ___________________________________
Student Signature Parent/Guardian Signature
 
"Hello! How do you do?"
"Ah, it's you, the moron."
Ignore the courtier and move on.
Pretend to be confused, or maybe looking for something.
 
Jones, Clinton W. "Point: The US Economy Would Suffer Greatly If The Eurozone Broke Up." Points Of View: European Debt Crisis (2013): 1. Points of View Reference Center. Web. 14 Feb. 2014.
 
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