Obviously I appreciate the massive amount of work this takes, and some design intentions may have to give way. But a few comments:
Internment camps - Social mobility instead of Imperialism
These are opposites. The social mobility tech is a "benevolent" government civic, while Imperialism is neutral and Internment Camps are "Malevolent". It would be very unfortunate to have this requirement, since they're thematically so opposite.
Maybe Internment camps could require Feudalism AND Imperialism? So have the arrow come from Feudalism, and have Imperialism as an extra requirement?
Arrakis transformation - sand farms instead of way of liet
This sounds fine, it looks like Way of Liet is an implicit requirement since that is required for water discipline?
Suk school - only Mind training instead of Mind training OR Genetic manipulation
Mentat logic - only Genetic manipulation instead of Mind training OR Genetic manipulation
Research labs - and instd of or in this - Mind training OR Genetic manipulation
These are also somewhat unfortunate.
The intention was that mind training and genetic manipulation be two different paths that you could follow to get things like Mentats or doctors. The Bene Gesserit and most factions would canonically favor mind training, but the Bene Tl would use genetic manipulation instead, to grow their twisted mentats rather than training new ones.
The Suk school change is fine, its not that big a deal.
But it looks like you *could* get the Mind training OR Genetic manipulation for Mentats if you shifted the genetic manipulation box up, to be horizontal with desert industry, moved the line from space guild to space ports up above this box, and then drew a line from mind training to mentat logic (and made this OR).
Then we could make Research Labs require Suk School OR Mentat logic, so it would still be possible to get to research labs *without* mind training by following the genetic manipulation path, or to get to reserach labs without mind training by following the mind training->suk school path.
Kindjal Blades should have an AND requirement with Personal shields; this was my omission for which I apologize. Multiple levels of melee should be prereqs.
Low energy vehicle should similarly be an AND requirement for Plas-steel armor; again my fault.
Improved Suspensors should be an AND requirement for Cooling systems - my fault.
And finally for aesthetics, we could consider cutting the tiers down by 2, condensing 18 into 17 and 20 into 19, the top of the tree is very very sparse.
Otherwise, this looks excellent.
We may have to make further minor changes due to playtesting, but otherwise I hope this is solid.