More South Americans, says I! Bring us Mapuche, Tupi or Brazil, which would, in my opinion, be more than 9000 times better than Canada, which would play out as a mix of England and USA, but lamer.
Though i guess Portugal goes before Brazil, in which case I say, bring us Mapuche and/or Tupi!
EDIT/PS: If Portugal was to be included in the game, would Rio be removed from the City States? I mean, it was a pretty important Portuguese city, and even the seat of the Portuguese royal family once, if I'm not mistaken. I know it was the seat of The Brazilian Empire's royal family.