Abstract. Data are presented on the biochemical findings in several internal organs from an Egyptian mummy with a 14C-dating of approximately 950 B.C. By use of radio immunoassay systems and gas chromatography/mass spectrometry, significant amounts of various drugs were detected in internal organs (lung, liver, stomach, intestines) as well as in hair, bone, skin/muscle and tendon. These analyses revealed a significant deposition of tetrahydrocannabinol (THC), nicotine (and its metabolite cotinine) and cocaine in the tissue from the mummy. The concentration profiles additionally provide evidence for the preferential ways of consumption: Thus, the highest levels of THC in lung specimens point to an inhalation of this drug - as it has been assumed from known ritual smoking ceremonies -, while nicotine and cocaine containing drugs showed their highest concentrations in the intestines and liver, so that they seem to have been consumed perorally. Furthermore, a histopathological examination of the internal organ tissues revealed some evidence for the underlying disease and the probable cause of death. Thus, a severe and presumably recurrent
intravital pulmonary bleeding, most obviously due to a parasitosis affecting the lung, was observed.
Introduction. At present, the consumption of significant amounts of drugs can be monitored by specific drug measurements or by analysis of their metabolites in various body fluids [1]. This procedure is usually applied to present drug addicts. For monitoring of patients, these techniques have been adapted to tissue specimens [2] and hair [3, 4]. In our previous studies we have used this methodology to analyse historic tissue samples [5-8]. Hence, we have provided circumstantial evidence that some drugs had already been consumed in historic populations. This investigation was done on historic skeletal remnants, either from pre-Columbian Peru or ancient Egypt. Skeletal tissue was of primary choice, as the skeletal material usually is quite resistant to postmortal autolysis. In this material, significant concentrations have been found of tetrahydrocannabinol, cocaine and nicotine [5, 6], as well as for caffeine [8]. The specificity of these measurements was evidenced by the selective presence of nicotine in skeletal material from skeletons from Middle Europe, while other drugs were absent [6]. In the present report, we describe the drug findings in an Egyptian mummy which had been unwrapped during the Munich Mummy Project [9]. These observations shed some significant light on historic medicine.
Materials and methods
Description of the mummy
The mummy presented in this report had been transferred to the Royal Bavarian Academy of Science in the 1820s by Dr. E W. Sieber, a private scientist from Prague (Czechia). According to 14C-dating analysis the mummy has been dated back to the 21st Egyptian dynasty (approx. 950 B.C.), although the exact source of the mummy has not been determined. A more detailed report on the mummy will be given elsewhere. During the unwrapping of the mummy which was performed due to the poor state of conservation (Fig. 1), the chest cavity revealed several small packages containing mummified organs which had been dried and then carefully wrapped in separate linen bindings. In four such packages small divine figurines were present. These represented the four sons of Horus which had also previously been found by SMITH and DAWSON [10] in lung, liver, stomach and intestine, so that an attribution of the packages to these organs could be performed (see Fig. 2). In addition, a macromorphological analysis of the organ remnants supported the aforementioned organ diagnoses, which were furthermore confirmed by histological analysis.
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